BACTALERT 3D was developed as an effective and sturdy solution for industrial sterility testing.
Bact Alert Blood Culture Bottles Registration Or UsagePlease be aware that we do not take any responsibility for accessing such information which may not comply with any legal process, regulation, registration or usage in the country of your origin.Best practice recommends collecting at least two sets of blood cultures prior to antimicrobial therapy to help improve organism recovery.
Bact Alert Blood Culture Bottles Trial Sterility TestingUnless otherwise noted, BD, the BD Logo and all other trademarks are the property of Becton, Dickinson and Company or its affiliates. Upon receipt, the bottle was visually inspected for growth, proper inoculation, and pertinent patient collection information. A total of 244 clinically significant isolates were recovered from 4,015 compliant pairs. Among the positive cultures, 170 (70) isolates were detected in both the BacTAlert PF and the Pedi-BacT bottles, while 47 (19) isolates were recovered in the BacTAlert PF bottle only and 27 (11) isolates were recovered in the Pedi-BacT bottle only. The BacTAlert PF bottle is a reliable blood culture bottle for pediatric blood culture specimens and may offer improved recovery of microbes from patients on antimicrobial therapy. The use of the nonvented bottle will both facilitate bottle processing and decrease expenditures for materials due to the elimination of the venting needles required for the original vented bottles. The performance of blood cultures from pediatric patients poses certain challenges not experienced when performing blood cultures for adult populations. Other factors include the fastidious nature of pediatric pathogens such as Streptococcus pneumoniae and Neisseria meningitidis, the changing venue of pathogens commonly retrieved from blood culture specimens, and the documentation of low concentrations of circulating organisms in the blood of some pediatric patients ( 2, 3, 4, 9 ). In response to these considerations, manufacturers of blood culture instruments have developed blood culture bottles for use exclusively in pediatric populations. These bottles differ from adult blood culture bottles in both formulation and volume of broth. Pediatric blood culture bottles are designed to hold approximately 20 ml of broth and accommodate an inoculation volume of up to 4 ml. Although adult blood culture bottles containing resins or charcoal-based substances for absorption of antimicrobial agents or other inhibitory substances were introduced several years ago, such additives were not available in a pediatric formulation. Calgary Laboratory Services (CLS), Calgary, Canada, is a centralized regional laboratory that provides microbiology services for both adult and pediatric patient populations, including the Alberta Childrens Hospital. Childrens Medical Center of Dallas, Dallas, Texas, (CMC) is a tertiary care pediatric teaching hospital. The broth formulation of the BacTAlert PF bottle is a casein soy base supplemented with brain heart infusion solids and other proteins with the addition of activated charcoal (8.5 wtvol) and 0.025 sodium polyethanolesulfonate. The Pedi-BacT bottle contains a media formulation that is composed of a brain heart infusion base with.020 sodium polyethanolesulfonate. The atmosphere of the BacTAlert PF bottle is oxygen and nitrogen, thus eliminating the need to vent the bottle. The content of the Pedi-BacT bottle is overlaid with carbon dioxide and nitrogen and requires venting prior to incubation. Unless otherwise designated, the methods used by both facilities for bottle processing were equivalent as established by the investigational study protocol. Blood from a single collection site was inoculated into each set of bottles. Individuals were instructed to inoculate the Pedi-BacT bottle first to ensure the performance of culture by the reference method. The optimum collection volume was set at 4.0 ml of blood for each bottle included in the evaluation. If a total of 8 ml was not collected, the blood was divided equally between the two bottles. Standard recommended antisepsis procedures were used during blood specimen collection.
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